Micro-scale blood plasma separation: from acoustophoresis to egg-beaters.

Micro-scale blood plasma separation: from acoustophoresis to egg-beaters Kersaudy-Kerhoas, Maïwenn; Sollier, Elodie

Egg beater as centrifuge: isolating human blood plasma from whole blood in resource-poor settings.

This paper demonstrates that a hand-powered egg beater can be modified to serve as a centrifuge for separating plasma from human whole blood. Immunoassays used to diagnose infectious diseases often require plasma from whole blood, and obtaining plasma typically requires electrically-powered centrifuges, which are not widely available in resource-limited settings. Human whole blood was loaded in...

Towards the Development of a Microfluidic Device for the Separation and Isolation of Circulating Tumor Cells from Whole Blood Using Acoustophoresis

We present progress towards the development of a microfluidic device with a novel design that assists in the separation and isolation of circulating tumor cells (CTCs) using acoustophoresis. A unique design is being developed to maximize the ‘filtering’ capability of the microfluidic device to eliminate the need to lyse red blood cells prior to isolation of CTCs. COMSOL modeling has allowed for...

Separation and Further Characterization of Hematopoietic Cell Populations Based on Phenotypic and Biophysical Properties Dykes, Josefina

Background: Excessive collection of platelets is an unwanted side effect in current centrifugation-based peripheral blood progenitor cell (PBPC) apheresis. We investigated a novel microchip-based acoustophoresis technique, utilizing ultrasonic standing wave forces for the removal of platelets from PBPC products. By applying an acoustic standing wave field onto a continuously flowing cell suspen...

Affinity-Bead-Mediated Enrichment of CD8+ Lymphocytes from Peripheral Blood Progenitor Cell Products Using Acoustophoresis

Acoustophoresis is a technique that applies ultrasonic standing wave forces in a microchannel to sort cells depending on their physical properties in relation to the surrounding media. Cell handling and separation for research and clinical applications aims to efficiently separate specific cell populations. Here, we investigated the sorting of CD8 lymphocytes from peripheral blood progenitor ce...